BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10150

Title: Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein

Authors: Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein"  . ., .-..

Assembly members:
SPCC24B10.08c protein, polymer, 58 residues, Formula weight is not available

Natural source:   Common Name: Fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
SPCC24B10.08c protein: GSSGSSGFDENWGADEELLL IDACETLGLGNWADIADYVG NARTKEECRDHYLKTYIE

Data sets:
Data typeCount
13C chemical shifts240
15N chemical shifts61
1H chemical shifts364

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SPCC24B10.08c protein1

Entities:

Entity 1, SPCC24B10.08c protein 58 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPHEASPGLU
2   ASNTRPGLYALAASPGLUGLULEULEULEU
3   ILEASPALACYSGLUTHRLEUGLYLEUGLY
4   ASNTRPALAASPILEALAASPTYRVALGLY
5   ASNALAARGTHRLYSGLUGLUCYSARGASP
6   HISTYRLEULYSTHRTYRILEGLU

Samples:

sample_1: SANT domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution & refinement

NMR spectrometers:

  • Bruker AVANCE2 900 MHz

Related Database Links:

PDB
EMBL CAB76217
REF NP_588011
SP Q9P7J7