BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10164

Title: Solution structure of the A1pp domain from human protein C6orf130

Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the A1pp domain from human protein C6orf130"  . ., .-..

Assembly members:
A1pp domain, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
A1pp domain: GSSGSSGGSRITYVKGDLFA CPKTDSLAHCISEDCRMGAG IAVLFKKKFGGVQELLNQQK KSGEVAVLKRDGRYIYYLIT KKRASHKPTYENLQKSLEAM KSHCLKNGVTDLSMPRIGCG LDRLQWENVSAMIEEVFEAT DIKITVYTL

Data sets:
Data typeCount
13C chemical shifts646
15N chemical shifts146
1H chemical shifts1029

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Uncharacterized protein C6orf1301

Entities:

Entity 1, Uncharacterized protein C6orf130 149 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYSERARG
2   ILETHRTYRVALLYSGLYASPLEUPHEALA
3   CYSPROLYSTHRASPSERLEUALAHISCYS
4   ILESERGLUASPCYSARGMETGLYALAGLY
5   ILEALAVALLEUPHELYSLYSLYSPHEGLY
6   GLYVALGLNGLULEULEUASNGLNGLNLYS
7   LYSSERGLYGLUVALALAVALLEULYSARG
8   ASPGLYARGTYRILETYRTYRLEUILETHR
9   LYSLYSARGALASERHISLYSPROTHRTYR
10   GLUASNLEUGLNLYSSERLEUGLUALAMET
11   LYSSERHISCYSLEULYSASNGLYVALTHR
12   ASPLEUSERMETPROARGILEGLYCYSGLY
13   LEUASPARGLEUGLNTRPGLUASNVALSER
14   ALAMETILEGLUGLUVALPHEGLUALATHR
15   ASPILELYSILETHRVALTYRTHRLEU

Samples:

sample_1: A1pp domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 15593 17421
PDB
DBJ BAF83628 BAG36161
GB AAH11709 AIC53444 AIC58012 EAX04011 EAX04012
REF NP_001181654 NP_659500 XP_001117219 XP_001117235 XP_001174030
SP Q9Y530