BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10165

Title: Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B

Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B"  . ., .-..

Assembly members:
CBM_21 domain, residues 8-156, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CBM_21 domain, residues 8-156: GSSGSSGAESESFVLDFSQP SADYLDFRNRLQADHVCLEN CVLKDKAIAGTVKVQNLAFE KTVKIRMTFDTWKSYTDFPC QYVKDTYAGSDRDTFSFDIS LPEKIQSYERMEFAVYYECN GQTYWDSNRGKNYRIIRAEL KSTQGMTKPHSGPDLG

Data sets:
Data typeCount
13C chemical shifts689
15N chemical shifts164
1H chemical shifts1053

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein phosphatase 1, regulatory (Inhibitor) subunit 3B1

Entities:

Entity 1, Protein phosphatase 1, regulatory (Inhibitor) subunit 3B 156 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAGLUSER
2   GLUSERPHEVALLEUASPPHESERGLNPRO
3   SERALAASPTYRLEUASPPHEARGASNARG
4   LEUGLNALAASPHISVALCYSLEUGLUASN
5   CYSVALLEULYSASPLYSALAILEALAGLY
6   THRVALLYSVALGLNASNLEUALAPHEGLU
7   LYSTHRVALLYSILEARGMETTHRPHEASP
8   THRTRPLYSSERTYRTHRASPPHEPROCYS
9   GLNTYRVALLYSASPTHRTYRALAGLYSER
10   ASPARGASPTHRPHESERPHEASPILESER
11   LEUPROGLULYSILEGLNSERTYRGLUARG
12   METGLUPHEALAVALTYRTYRGLUCYSASN
13   GLYGLNTHRTYRTRPASPSERASNARGGLY
14   LYSASNTYRARGILEILEARGALAGLULEU
15   LYSSERTHRGLNGLYMETTHRLYSPROHIS
16   SERGLYPROASPLEUGLY

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE II 900 MHz

Related Database Links:

PDB
DBJ BAB14811 BAG53215
GB AAH43388 ADQ33125 AIC57095 EAW65572 EHH63994
REF NP_001188258 NP_078883 XP_003902491 XP_003902492 XP_004046665
SP Q86XI6