BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10193

Title: Solution structure of the Myb-like DNA-binding domain of human ZZZ3 protein

Authors: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Myb-like DNA-binding domain of human ZZZ3 protein"  . ., .-..

Assembly members:
Myb-like DNA-binding domain, UNP residues 652-713, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Myb-like DNA-binding domain, UNP residues 652-713: GSSGSSGNQLWTVEEQKKLE QLLIKYPPEEVESRRWQKIA DELGNRTAKQVASQVQKYFI KLTKAGIPVSGPSSG

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts70
1H chemical shifts497

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Myb-like DNA-binding domain, UNP residues 652-7131

Entities:

Entity 1, Myb-like DNA-binding domain, UNP residues 652-713 75 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNGLNLEU
2   TRPTHRVALGLUGLUGLNLYSLYSLEUGLU
3   GLNLEULEUILELYSTYRPROPROGLUGLU
4   VALGLUSERARGARGTRPGLNLYSILEALA
5   ASPGLULEUGLYASNARGTHRALALYSGLN
6   VALALASERGLNVALGLNLYSTYRPHEILE
7   LYSLEUTHRLYSALAGLYILEPROVALSER
8   GLYPROSERSERGLY

Samples:

sample_1: Myb-like DNA-binding domain, UNP residues 652-713, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB AAH35079 ADZ15730 AIC62127