BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10194

Title: Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3

Authors: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3"  . ., .-..

Assembly members:
SH3 domain, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
SH3 domain: GSSGSSGRRAKALLDFERHD DDELGFRKNDIITIISQKDE HCWVGELNGLRGWFPAKFVE VLDERSKEYSIASGPSSG

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts72
1H chemical shifts492

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 78 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGARGALA
2   LYSALALEULEUASPPHEGLUARGHISASP
3   ASPASPGLULEUGLYPHEARGLYSASNASP
4   ILEILETHRILEILESERGLNLYSASPGLU
5   HISCYSTRPVALGLYGLULEUASNGLYLEU
6   ARGGLYTRPPHEPROALALYSPHEVALGLU
7   VALLEUASPGLUARGSERLYSGLUTYRSER
8   ILEALASERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.00 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC82539 BAE42041
EMBL CAG04108
GB AAH18197 AAH24122 AAH25561 AAH54597 AAH62060
REF NP_001070013 NP_598852 NP_942082 NP_998495 XP_001097960
SP Q2KI13 Q6P6R7 Q7T2D0 Q8VCZ6
TPG DAA29091