BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10212

Title: Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)

Authors: Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)"  . ., .-..

Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
SH3 domain: GSSGSSGPLPNPEGLDSDFL AVLSDYPSPDISPPIFRRGE KLRVISDEGGWWKAISLSTG RESYIPGICVARVSGPSSG

Data sets:
Data typeCount
13C chemical shifts304
15N chemical shifts68
1H chemical shifts475

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 79 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLEUPRO
2   ASNPROGLUGLYLEUASPSERASPPHELEU
3   ALAVALLEUSERASPTYRPROSERPROASP
4   ILESERPROPROILEPHEARGARGGLYGLU
5   LYSLEUARGVALILESERASPGLUGLYGLY
6   TRPTRPLYSALAILESERLEUSERTHRGLY
7   ARGGLUSERTYRILEPROGLYILECYSVAL
8   ALAARGVALSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.28 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0,4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAA13758 BAE89471 BAG35478 BAG73760 BAH12578
EMBL CAB53536 CAG38774
GB AAC27662 AAC50357 AAH07042 AAV38795 AAV38796
PRF 2204265A
REF NP_001039021 NP_001039022 NP_001269893 NP_001274231 NP_006739
SP Q13239