BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10235

Title: Solution Structure of the C-terminal Phosphotyrosine Interaction Domain of APBB2 from Mouse   PubMed: 18650440

Authors: Li, H.; Hayashi, F.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Koshiba, S.; Hayashi, F.; Tochio, N.; Tomozawa, T.; Kasai, T.; Yabuki, T.; Motoda, Y.; Harada, T.; Watanabe, S.; Inoue, M.; Hayashizaki, Y.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Structure of the C-terminal PID Domain of Fe65L1 Complexed with the Cytoplasmic Tail of APP Reveals a Novel Peptide Binding Mode"  J. Biol. Chem. 283, 27165-27178 (2008).

Assembly members:
Phosphotyrosine-Interaction Domain, polymer, 136 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Phosphotyrosine-Interaction Domain: GSSGSSGPTPKTELVQKFRV QYLGMLPVDRPVGMDTLNSA IENLMTSSSKEDWPSVNMNV ADATVTVISEKNEEEVLVEC RVRFLSFMGVGKDVHTFAFI MDTGNQRFECHVFWCEPNAA NVSEAVQAACSGPSSG

Data sets:
Data typeCount
13C chemical shifts567
15N chemical shifts136
1H chemical shifts891

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Phosphotyrosine-Interaction Domain1

Entities:

Entity 1, Phosphotyrosine-Interaction Domain 136 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROTHRPRO
2   LYSTHRGLULEUVALGLNLYSPHEARGVAL
3   GLNTYRLEUGLYMETLEUPROVALASPARG
4   PROVALGLYMETASPTHRLEUASNSERALA
5   ILEGLUASNLEUMETTHRSERSERSERLYS
6   GLUASPTRPPROSERVALASNMETASNVAL
7   ALAASPALATHRVALTHRVALILESERGLU
8   LYSASNGLUGLUGLUVALLEUVALGLUCYS
9   ARGVALARGPHELEUSERPHEMETGLYVAL
10   GLYLYSASPVALHISTHRPHEALAPHEILE
11   METASPTHRGLYASNGLNARGPHEGLUCYS
12   HISVALPHETRPCYSGLUPROASNALAALA
13   ASNVALSERGLUALAVALGLNALAALACYS
14   SERGLYPROSERSERGLY

Samples:

sample_1: Phosphotyrosine-Interaction Domain, [U-13C; U-15N], 0.84 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 2 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1c, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.897, Kobayashi, N. - data analysis

CYANA v1.0.7, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 900 MHz

Related Database Links:

BMRB 10237 10238 10239
PDB
DBJ BAB23568 BAE31851 BAE39700 BAE41482 BAE42990
GB AAD55360 AAH76587 EDL37768 EDL90042 EGW02447
REF NP_001188342 NP_001188343 NP_001188344 NP_001188345 NP_001297555
SP Q9DBR4