BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10240

Title: Solution structures of the SH3 domain of human Src substrate cortactin

Authors: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human Src substrate cortactin"  . ., .-..

Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
SH3 domain: GSSGSSGTYDEYENDLGITA VALYDYQAAGDDEISFDPDD IITNIEMIDDGWWRGVCKGR YGLFPANYVELRQSGPSSG

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts79
1H chemical shifts486

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 79 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRTYRASP
2   GLUTYRGLUASNASPLEUGLYILETHRALA
3   VALALALEUTYRASPTYRGLNALAALAGLY
4   ASPASPGLUILESERPHEASPPROASPASP
5   ILEILETHRASNILEGLUMETILEASPASP
6   GLYTRPTRPARGGLYVALCYSLYSGLYARG
7   TYRGLYLEUPHEPROALAASNTYRVALGLU
8   LEUARGGLNSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAD96333 BAE02409 BAF83786 BAG52416 BAG65370
EMBL CAH93452
GB AAA49031 AAA58455 AAB26248 AAH08799 AAH33889
REF NP_001068755 NP_001127021 NP_001171669 NP_001253213 NP_005222
SP Q01406 Q14247
TPG DAA13535