BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10244

Title: Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462

Deposition date: 2008-10-24 Original release date: 2009-11-03

Authors: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462"  . ., .-..

Assembly members:
C2H2 type zinc finger domain, polymer, 88 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
C2H2 type zinc finger domain: GSSGSSGLKRDFIILGNGPR LQNSTYQCKHCDSKLQSTAE LTSHLNIHNEEFQKRAKRQE RRKQLLSKQKYADGAFADFK QESGPSSG

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts77
1H chemical shifts500

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2H2 type zinc finger domain1
2ZINC ION2

Entities:

Entity 1, C2H2 type zinc finger domain 88 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEULYSARG
2   ASPPHEILEILELEUGLYASNGLYPROARG
3   LEUGLNASNSERTHRTYRGLNCYSLYSHIS
4   CYSASPSERLYSLEUGLNSERTHRALAGLU
5   LEUTHRSERHISLEUASNILEHISASNGLU
6   GLUPHEGLNLYSARGALALYSARGGLNGLU
7   ARGARGLYSGLNLEULEUSERLYSGLNLYS
8   TYRALAASPGLYALAPHEALAASPPHELYS
9   GLNGLUSERGLYPROSERSERGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C2H2 type zinc finger domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; NTA 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB47432 BAG11361
EMBL CAH18698 CAH56168
GB AAH36884 AAI72445 AIC60541 EAW59010 EAW59011
REF NP_067047 XP_001109263 XP_002820132 XP_003260371 XP_003810950
SP Q96JM2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts