BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10246

Title: Solution structures of the C2H2 type zinc finger domain of human Transcriptional repressor CTCF

Authors: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the C2H2 type zinc finger domain of human Transcriptional repressor CTCF"  . ., .-..

Assembly members:
C2H2 type zinc finger domain, polymer, 86 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
C2H2 type zinc finger domain: GSSGSSGRTHTGEKPYACSH CDKTFRQKQLLDMHFKRYHD PNFVPAAFVCSKCGKTFTRR NTMARHADNCAGPDGVEGEN SGPSSG

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts70
1H chemical shifts487

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Transcriptional repressor CTCF1
2ZINC ION No.12
3ZINC ION No.22

Entities:

Entity 1, Transcriptional repressor CTCF 86 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGTHRHIS
2   THRGLYGLULYSPROTYRALACYSSERHIS
3   CYSASPLYSTHRPHEARGGLNLYSGLNLEU
4   LEUASPMETHISPHELYSARGTYRHISASP
5   PROASNPHEVALPROALAALAPHEVALCYS
6   SERLYSCYSGLYLYSTHRPHETHRARGARG
7   ASNTHRMETALAARGHISALAASPASNCYS
8   ALAGLYPROASPGLYVALGLUGLYGLUASN
9   SERGLYPROSERSERGLY

Entity 2, ZINC ION No.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C2H2 type zinc finger domain, [U-13C; U-15N], 1.5 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; ZnCl2 10 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAE88629 BAE90237
REF XP_003917105 XP_009194954