BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10248

Title: Solution Structure of the HMG_box Domain of Murine Bobby Sox Homolog

Authors: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the HMG_box Domain of Murine Bobby Sox Homolog"  . ., .-..

Assembly members:
HMG_box domain, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
HMG_box domain: GSSGSSGARRPMNAFLLFCK RHRSLVRQEHPRLDNRGATK ILADWWAVLDPKEKQKYTDM AKEYKDAFMKANPGYRSGPS SG

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts68
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG_box domain1

Entities:

Entity 1, HMG_box domain 82 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAARGARG
2   PROMETASNALAPHELEULEUPHECYSLYS
3   ARGHISARGSERLEUVALARGGLNGLUHIS
4   PROARGLEUASPASNARGGLYALATHRLYS
5   ILELEUALAASPTRPTRPALAVALLEUASP
6   PROLYSGLULYSGLNLYSTYRTHRASPMET
7   ALALYSGLUTYRLYSASPALAPHEMETLYS
8   ALAASNPROGLYTYRARGSERGLYPROSER
9   SERGLY

Samples:

sample_1: HMG_box domain, [U-13C; U-15N], 1.55 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v1.0.7, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB ELK18141 ELV09777
REF XP_006905507 XP_011383451 XP_011383519 XP_011383589 XP_011383651