BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10252

Title: Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse

Authors: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse"  . ., .-..

Assembly members:
HMG box, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
HMG box: GSSGSSGKKKKKKDPNEPQK PVSAYALFFRDTQAAIKGQN PNATFGEVSKIVASMWDGLG EEQKQVYKKKTEAAKKEYLK QLAAYRASLVSKSYTDSGPS SG

Data sets:
Data typeCount
13C chemical shifts394
15N chemical shifts90
1H chemical shifts599

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG box1

Entities:

Entity 1, HMG box 102 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSLYSLYS
2   LYSLYSLYSASPPROASNGLUPROGLNLYS
3   PROVALSERALATYRALALEUPHEPHEARG
4   ASPTHRGLNALAALAILELYSGLYGLNASN
5   PROASNALATHRPHEGLYGLUVALSERLYS
6   ILEVALALASERMETTRPASPGLYLEUGLY
7   GLUGLUGLNLYSGLNVALTYRLYSLYSLYS
8   THRGLUALAALALYSLYSGLUTYRLEULYS
9   GLNLEUALAALATYRARGALASERLEUVAL
10   SERLYSSERTYRTHRASPSERGLYPROSER
11   SERGLY

Samples:

sample_1: HMG_box domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA34528 BAC26568 BAC30091 BAG11241 BAG63663
EMBL CAF93289 CBY16474
GB AAH16665 AAH80732 AAI47900 AAI66269 AAL78656
REF NP_001095566 NP_001102124 NP_001121484 NP_001248418 NP_055544
SP O94900 Q66JW3
TPG DAA22741