BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10261

Title: Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase

Authors: Sato, M.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase"  . ., .-..

Assembly members:
WHEP-TRS domain, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
WHEP-TRS domain: GSSGSSGTTAKPQQIQALMD EVTKQGNIVRELKAQKADKN EVAAEVAKLLDLKKQLAVAE GKPPEAPKGKKKKSGPSSG

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts69
1H chemical shifts480

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WHEP-TRS domain1

Entities:

Entity 1, WHEP-TRS domain 79 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRTHRALA
2   LYSPROGLNGLNILEGLNALALEUMETASP
3   GLUVALTHRLYSGLNGLYASNILEVALARG
4   GLULEULYSALAGLNLYSALAASPLYSASN
5   GLUVALALAALAGLUVALALALYSLEULEU
6   ASPLEULYSLYSGLNLEUALAVALALAGLU
7   GLYLYSPROPROGLUALAPROLYSGLYLYS
8   LYSLYSLYSSERGLYPROSERSERGLY

Samples:

sample_1: WHWP-TRS domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAA95668 BAD96487 BAF83546 BAJ20957
EMBL CAA64381 CAA89153
GB AAH02384 AAH06328 AAH11639 AAH11849 AAH15011
REF NP_001253877 NP_004981 XP_002823470 XP_004053501 XP_005571392
SP P56192