BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10264

Title: The solution structure of the VHS domain of human Signal transducing adaptor molecule 2

Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the VHS domain of human Signal transducing adaptor molecule 2"  . ., .-..

Assembly members:
VHS domain, polymer, 163 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
VHS domain: GSSGSSGMPLFTANPFEQDV EKATNEYNTTEDWSLIMDIC DKVGSTPNGAKDCLKAIMKR VNHKVPHVALQALTLLGACV ANCGKIFHLEVCSRDFATEV RAVIKNKAHPKVCEKLKSLM VEWSEEFQKDPQFSLISATI KSMKEEGITFPPAGSQTSGP SSG

Data sets:
Data typeCount
13C chemical shifts703
15N chemical shifts162
1H chemical shifts1054

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VHS domain1

Entities:

Entity 1, VHS domain 163 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETPROLEU
2   PHETHRALAASNPROPHEGLUGLNASPVAL
3   GLULYSALATHRASNGLUTYRASNTHRTHR
4   GLUASPTRPSERLEUILEMETASPILECYS
5   ASPLYSVALGLYSERTHRPROASNGLYALA
6   LYSASPCYSLEULYSALAILEMETLYSARG
7   VALASNHISLYSVALPROHISVALALALEU
8   GLNALALEUTHRLEULEUGLYALACYSVAL
9   ALAASNCYSGLYLYSILEPHEHISLEUGLU
10   VALCYSSERARGASPPHEALATHRGLUVAL
11   ARGALAVALILELYSASNLYSALAHISPRO
12   LYSVALCYSGLULYSLEULYSSERLEUMET
13   VALGLUTRPSERGLUGLUPHEGLNLYSASP
14   PROGLNPHESERLEUILESERALATHRILE
15   LYSSERMETLYSGLUGLUGLYILETHRPHE
16   PROPROALAGLYSERGLNTHRSERGLYPRO
17   SERSERGLY

Samples:

sample_1: VHS domain, [U-13C; U-15N], 1.5 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.926, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

BMRB 18185
PDB
DBJ BAF84954 BAF85536 BAG72771 BAK62690
EMBL CAB63735 CAL38297
GB AAC63963 AAC63964 AAH28740 AAH83912 AAI12605
REF NP_001012085 NP_001069574 NP_001267210 NP_005834 XP_002749486
SP O75886 Q5XHY7
TPG DAA32691