BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10267

Title: Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1

Authors: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1"  . ., .-..

Assembly members:
PDZ domain, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
PDZ domain: GSSGSSGMASTFNPRECKLS KQEGQNYGFFLRIEKDTDGH LIRVIEEGSPAEKAGLLDGD RVLRINGVFVDKEEHAQVVE LVRKSGNSVTLLVLDGDSYE KAVKNQVDLKELDQ

Data sets:
Data typeCount
13C chemical shifts481
15N chemical shifts120
1H chemical shifts773

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALASER
2   THRPHEASNPROARGGLUCYSLYSLEUSER
3   LYSGLNGLUGLYGLNASNTYRGLYPHEPHE
4   LEUARGILEGLULYSASPTHRASPGLYHIS
5   LEUILEARGVALILEGLUGLUGLYSERPRO
6   ALAGLULYSALAGLYLEULEUASPGLYASP
7   ARGVALLEUARGILEASNGLYVALPHEVAL
8   ASPLYSGLUGLUHISALAGLNVALVALGLU
9   LEUVALARGLYSSERGLYASNSERVALTHR
10   LEULEUVALLEUASPGLYASPSERTYRGLU
11   LYSALAVALLYSASNGLNVALASPLEULYS
12   GLULEUASPGLN

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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