BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10268

Title: Solution structures of the CA domain of human protocadherin 9

Authors: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the CA domain of human protocadherin 9"  . ., .-..

Assembly members:
CA domain, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CA domain: GSSGSSGNDNRPVFKEGQVE VHIPENAPVGTSVIQLHATD ADIGSNAEIRYIFGAQVAPA TKRLFALNNTTGLITVQRSL DREETAIHKVTVLASDGSST PARATVTINVTDVN

Data sets:
Data typeCount
13C chemical shifts469
15N chemical shifts119
1H chemical shifts753

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CA domain1

Entities:

Entity 1, CA domain 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNASPASN
2   ARGPROVALPHELYSGLUGLYGLNVALGLU
3   VALHISILEPROGLUASNALAPROVALGLY
4   THRSERVALILEGLNLEUHISALATHRASP
5   ALAASPILEGLYSERASNALAGLUILEARG
6   TYRILEPHEGLYALAGLNVALALAPROALA
7   THRLYSARGLEUPHEALALEUASNASNTHR
8   THRGLYLEUILETHRVALGLNARGSERLEU
9   ASPARGGLUGLUTHRALAILEHISLYSVAL
10   THRVALLEUALASERASPGLYSERSERTHR
11   PROALAARGALATHRVALTHRILEASNVAL
12   THRASPVALASN

Samples:

sample_1: CA domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB