BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10269

Title: Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4

Authors: Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4"  . ., .-..

Assembly members:
Chromo domain, polymer, 64 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Chromo domain: GSSGSSGKPEWMMIHRILNH SVDKKGHVHYLIKWRDLPYD QASWESEDVEIQDYDLFKQS YWNH

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts65
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chromo domain1

Entities:

Entity 1, Chromo domain 64 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSPROGLU
2   TRPMETMETILEHISARGILELEUASNHIS
3   SERVALASPLYSLYSGLYHISVALHISTYR
4   LEUILELYSTRPARGASPLEUPROTYRASP
5   GLNALASERTRPGLUSERGLUASPVALGLU
6   ILEGLNASPTYRASPLEUPHELYSGLNSER
7   TYRTRPASNHIS

Samples:

sample_1: Chromo domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC28749 BAD90499 BAE32198 BAG54725
EMBL CAA60384
GB AAH38596 AAH58578 ABZ92034 EAW88777 EAW88778
REF NP_001193430 NP_001264 NP_001284482 NP_666091 XP_001063352
SP Q14839 Q6PDQ2
TPG DAA29256