BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10276

Title: Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse

Authors: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse"  . ., .-..

Assembly members:
Tudor domain, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Tudor domain: GSSGSSGKVWKPGDECFALY WEDNKFYRAEVEALHSSGMT AVVKFTDYGNYEEVLLSNIK PVQTEAWVRDPNSGPSSG

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts74
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tudor domain1

Entities:

Entity 1, Tudor domain 78 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSVALTRP
2   LYSPROGLYASPGLUCYSPHEALALEUTYR
3   TRPGLUASPASNLYSPHETYRARGALAGLU
4   VALGLUALALEUHISSERSERGLYMETTHR
5   ALAVALVALLYSPHETHRASPTYRGLYASN
6   TYRGLUGLUVALLEULEUSERASNILELYS
7   PROVALGLNTHRGLUALATRPVALARGASP
8   PROASNSERGLYPROSERSERGLY

Samples:

sample_1: Tudor domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC26033 BAC28263
GB AAH05670 AAH66144 EDL35743
REF NP_001240684 NP_766193 XP_006518972 XP_006518973 XP_006518974
SP Q91W18