BMRB Entry 10276
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10276
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse
Deposition date: 2008-12-15 Original release date: 2009-12-14
Authors: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse" . ., .-..
Assembly members:
Tudor domain, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Tudor domain: GSSGSSGKVWKPGDECFALY
WEDNKFYRAEVEALHSSGMT
AVVKFTDYGNYEEVLLSNIK
PVQTEAWVRDPNSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 484 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tudor domain | 1 |
Entities:
Entity 1, Tudor domain 78 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | VAL | TRP | ||||
| 2 | LYS | PRO | GLY | ASP | GLU | CYS | PHE | ALA | LEU | TYR | ||||
| 3 | TRP | GLU | ASP | ASN | LYS | PHE | TYR | ARG | ALA | GLU | ||||
| 4 | VAL | GLU | ALA | LEU | HIS | SER | SER | GLY | MET | THR | ||||
| 5 | ALA | VAL | VAL | LYS | PHE | THR | ASP | TYR | GLY | ASN | ||||
| 6 | TYR | GLU | GLU | VAL | LEU | LEU | SER | ASN | ILE | LYS | ||||
| 7 | PRO | VAL | GLN | THR | GLU | ALA | TRP | VAL | ARG | ASP | ||||
| 8 | PRO | ASN | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Tudor domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC26033 BAC28263 |
| GB | AAH05670 AAH66144 EDL35743 |
| REF | NP_001240684 NP_766193 XP_006518972 XP_006518973 XP_006518974 |
| SP | Q91W18 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts