BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10281

Title: Solution structure of the homeobox domain of the human paired box protein Pax-6

Authors: Ohnishi, S.; Kigawa, T.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Ohnishi, S.; Kigawa, T.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of the human paired box protein Pax-6"  . ., .-..

Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeobox domain: GSSGSSGQRNRTSFTQEQIE ALEKEFERTHYPDVFARERL AAKIDLPEARIQVWFSNRRA KWRREEKLRNQRRQSGPSSG

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts70
1H chemical shifts485

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNARGASN
2   ARGTHRSERPHETHRGLNGLUGLNILEGLU
3   ALALEUGLULYSGLUPHEGLUARGTHRHIS
4   TYRPROASPVALPHEALAARGGLUARGLEU
5   ALAALALYSILEASPLEUPROGLUALAARG
6   ILEGLNVALTRPPHESERASNARGARGALA
7   LYSTRPARGARGGLUGLULYSLEUARGASN
8   GLNARGARGGLNSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.50 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert. P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA20936 BAA23004 BAA24024 BAB62531 BAC25729
EMBL CAA04395 CAA11364 CAA11365 CAA11366 CAA11367
GB AAA36416 AAA40109 AAA59962 AAB05932 AAB07733
PIR S37689
PRF 1717390A 1902328A 1911405A
REF NP_000271 NP_001006763 NP_001035735 NP_001075686 NP_001079413
SP O73917 P26367 P26630 P47237 P47238
TPG DAA21851