BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10293

Title: Solution structure of the homeobox domain of LIM/homeobox protein Lhx9

Authors: Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain of LIM/homeobox protein Lhx9"  . ., .-..

Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeobox domain: GSSGSSGKRMRTSFKHHQLR TMKSYFAINHNPDAKDLKQL AQKTGLTKRVLQVWFQNARA KFRRNLLRQENGGVSGPSSG

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts73
1H chemical shifts481

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSARGMET
2   ARGTHRSERPHELYSHISHISGLNLEUARG
3   THRMETLYSSERTYRPHEALAILEASNHIS
4   ASNPROASPALALYSASPLEULYSGLNLEU
5   ALAGLNLYSTHRGLYLEUTHRLYSARGVAL
6   LEUGLNVALTRPPHEGLNASNALAARGALA
7   LYSPHEARGARGASNLEULEUARGGLNGLU
8   ASNGLYGLYVALSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA21846 BAC04371 BAC35432 BAE45355 BAF46217
EMBL CAB59907 CAB59908 CAB59909 CAB97493 CAB98128
GB AAA50258 AAB08752 AAD20012 AAD20013 AAD22008
REF NP_001014434 NP_001017710 NP_001019715 NP_001020736 NP_001032320
SP A0JNI8 A2I8Z7 P50458 Q1LWV4 Q68EY3
TPG DAA20966 DAA24308