BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10295

Title: Solution structure of the homeobox domain of Homeobox protein BarH-like 1

Authors: Ohnishi, S.; Yoneyama, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Ohnishi, S.; Yoneyama, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain of Homeobox protein BarH-like 1"  . ., .-..

Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeobox domain: GSSGSSGGEPGTKAKKGRRS RTVFTELQLMGLEKRFEKQK YLSTPDRIDLAESLGLSQLQ VKTWYQNRRMKWKKSGPSSG

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts70
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYGLUPRO
2   GLYTHRLYSALALYSLYSGLYARGARGSER
3   ARGTHRVALPHETHRGLULEUGLNLEUMET
4   GLYLEUGLULYSARGPHEGLULYSGLNLYS
5   TYRLEUSERTHRPROASPARGILEASPLEU
6   ALAGLUSERLEUGLYLEUSERGLNLEUGLN
7   VALLYSTHRTRPTYRGLNASNARGARGMET
8   LYSTRPLYSLYSSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAO79524
EMBL CAA69257 CAC10357
GB AAD21043 AAG18573 AAG23738 AAH09458 AAH64363
REF NP_001102350 NP_031552 NP_067545 XP_001109954 XP_001507934
SP Q9ER42 Q9HBU1 Q9W6D8