BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10297

Title: Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8

Authors: Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.

Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8"  . ., .-..

Assembly members:
homeobox domain, polymer, 64 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeobox domain: GSSGSSGQPPLKNLLSLLKA YYALNAQPSAEELSKIADSV NLPLDVVKKWFEKMQAGQIS VQSS

Data sets:
Data typeCount
13C chemical shifts264
15N chemical shifts62
1H chemical shifts418

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 64 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNPROPRO
2   LEULYSASNLEULEUSERLEULEULYSALA
3   TYRTYRALALEUASNALAGLNPROSERALA
4   GLUGLULEUSERLYSILEALAASPSERVAL
5   ASNLEUPROLEUASPVALVALLYSLYSTRP
6   PHEGLULYSMETGLNALAGLYGLNILESER
7   VALGLNSERSER

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAA03259 BAA11178
GB EMC89439 EMP31625 EOA95468 KFM04765 KFO63461
REF NP_990462 XP_005027918 XP_005027919 XP_005027920 XP_005040695
SP P36197