BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10298

Title: structure of the third Homeodomain from the human homeobox and leucine zipper protein, Homez

Authors: Ohnishi, S.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.

Citation: Ohnishi, S.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "structure of the third Homeodomain from the human homeobox and leucine zipper protein, Homez"  . ., .-..

Assembly members:
homeodomain, polymer, 70 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeodomain: GSSGSSGPLPIPPPPPDIQP LERYWAAHQQLRETDIPQLS QASRLSTQQVLDWFDSRLPQ PAEVSGPSSG

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts62
1H chemical shifts417

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeodomain1

Entities:

Entity 1, homeodomain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLEUPRO
2   ILEPROPROPROPROPROASPILEGLNPRO
3   LEUGLUARGTYRTRPALAALAHISGLNGLN
4   LEUARGGLUTHRASPILEPROGLNLEUSER
5   GLNALASERARGLEUSERTHRGLNGLNVAL
6   LEUASPTRPPHEASPSERARGLEUPROGLN
7   PROALAGLUVALSERGLYPROSERSERGLY

Samples:

sample_1: homeodomain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA92681 BAG10069 BAG59479 BAG63292 BAG63992
GB AAI30393 AAN76991 AIC60498 EAW66175 EAW66176
REF NP_065885 XP_001150591 XP_002824626 XP_003808144 XP_003924361
SP Q8IX15