BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10306

Title: Solution structure of the 1st zf-C2H2 domain from Human B-cell lymphoma 6 protein

Authors: Tomizawa, T.; Sato, M.; Saito, K.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Sato, M.; Saito, K.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 1st zf-C2H2 domain from Human B-cell lymphoma 6 protein"  . ., .-..

Assembly members:
zf-C2H2 domain, polymer, 43 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell-free synthesis

Entity Sequences (FASTA):
zf-C2H2 domain: GSSGSSGNGAFFCNECDCRF SEEASLKRHTLQTHSDKSGP SSG

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts35
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-C2H2 domain1
2ZINC ION2

Entities:

Entity 1, zf-C2H2 domain 43 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNGLYALA
2   PHEPHECYSASNGLUCYSASPCYSARGPHE
3   SERGLUGLUALASERLEULYSARGHISTHR
4   LEUGLNTHRHISSERASPLYSSERGLYPRO
5   SERSERGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zf-C2H2 domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9825, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_012383937