BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10317

Title: Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human

Authors: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human""  . ., .-..

Assembly members:
PH domain, polymer, 128 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
PH domain: GSSGSSGKRSNSIKRNPNAP VVRRGWLYKQDSTGMKLWKK RWFVLSDLCLFYYRDEKEEG ILGSILLPSFQIALLTSEDH INRKYAFKAAHPNMRTYYFC TDTGKEMELWMKAMLDAALV QTSGPSSG

Data sets:
Data typeCount
13C chemical shifts558
15N chemical shifts117
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pleckstrin homology domain-containing family A member 51

Entities:

Entity 1, Pleckstrin homology domain-containing family A member 5 128 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSARGSER
2   ASNSERILELYSARGASNPROASNALAPRO
3   VALVALARGARGGLYTRPLEUTYRLYSGLN
4   ASPSERTHRGLYMETLYSLEUTRPLYSLYS
5   ARGTRPPHEVALLEUSERASPLEUCYSLEU
6   PHETYRTYRARGASPGLULYSGLUGLUGLY
7   ILELEUGLYSERILELEULEUPROSERPHE
8   GLNILEALALEULEUTHRSERGLUASPHIS
9   ILEASNARGLYSTYRALAPHELYSALAALA
10   HISPROASNMETARGTHRTYRTYRPHECYS
11   THRASPTHRGLYLYSGLUMETGLULEUTRP
12   METLYSALAMETLEUASPALAALALEUVAL
13   GLNTHRSERGLYPROSERSERGLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB21777 BAC98233 BAG10115 BAG57882 BAG58167
GB AAG22817 AAH00969 AAH70174 AAI27093 AAM44231
REF NP_001137293 NP_001243399 NP_001243716 NP_061885 NP_647556
SP Q9HAU0
TPG DAA29392