BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10318

Title: Solution structure of the PTB domain of KIAA1075 protein from human

Authors: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PTB domain of KIAA1075 protein from human"  . ., .-..

Assembly members:
PTB domain, polymer, 160 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
PTB domain: GSSGSSGMSTAADLLRQGAA CSVLYLTSVETESLTGPQAV ARASSAALSCSPRPTPAVVH FKVSAQGITLTDNQRKLFFR RHYPVNSITFSSTDPQDRRW TNPDGTTSKIFGFVAKKPGS PWENVCHLFAELDPDQPAGA IVTFITKVLLGQRKSGPSSG

Data sets:
Data typeCount
13C chemical shifts653
15N chemical shifts148
1H chemical shifts1031

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PTB domain1

Entities:

Entity 1, PTB domain 160 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETSERTHR
2   ALAALAASPLEULEUARGGLNGLYALAALA
3   CYSSERVALLEUTYRLEUTHRSERVALGLU
4   THRGLUSERLEUTHRGLYPROGLNALAVAL
5   ALAARGALASERSERALAALALEUSERCYS
6   SERPROARGPROTHRPROALAVALVALHIS
7   PHELYSVALSERALAGLNGLYILETHRLEU
8   THRASPASNGLNARGLYSLEUPHEPHEARG
9   ARGHISTYRPROVALASNSERILETHRPHE
10   SERSERTHRASPPROGLNASPARGARGTRP
11   THRASNPROASPGLYTHRTHRSERLYSILE
12   PHEGLYPHEVALALALYSLYSPROGLYSER
13   PROTRPGLUASNVALCYSHISLEUPHEALA
14   GLULEUASPPROASPGLNPROALAGLYALA
15   ILEVALTHRPHEILETHRLYSVALLEULEU
16   GLYGLNARGLYSSERGLYPROSERSERGLY

Samples:

sample_1: PTB domain, [U-13C; U-15N], 0.57 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

BMRB 17364
PDB
DBJ BAA83027 BAG09960 BAG54765
EMBL CAB70815 CAH56176
GB AAH54099 AAI10855 AAI29829 AAI29830 AAI31504
REF NP_056134 NP_736610 NP_938072 XP_001102202 XP_001790253
SP Q63HR2
TPG DAA29990