BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10337

Title: Solution structure of SH3 domain in Suppressor of T-cell receptor signaling 1

Authors: Sukegawa, S.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation: Sukegawa, S.; Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of SH3 domain in Suppressor of T-cell receptor signaling 1"  . ., .-..

Assembly members:
SH3 domain, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli

Entity Sequences (FASTA):
SH3 domain: GSSGSSGSRDIRFANHETLQ VIYPYTPQNDDELELVPGDF IFMSPMEQTSTSEGWIYGTS LTTGCSGLLPENYITKADEC STWIFHGSSGPSSG

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts79
1H chemical shifts539

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 94 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERARGASP
2   ILEARGPHEALAASNHISGLUTHRLEUGLN
3   VALILETYRPROTYRTHRPROGLNASNASP
4   ASPGLULEUGLULEUVALPROGLYASPPHE
5   ILEPHEMETSERPROMETGLUGLNTHRSER
6   THRSERGLUGLYTRPILETYRGLYTHRSER
7   LEUTHRTHRGLYCYSSERGLYLEULEUPRO
8   GLUASNTYRILETHRLYSALAASPGLUCYS
9   SERTHRTRPILEPHEHISGLYSERSERGLY
10   PROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB85545 BAC11468 BAC25403 BAC28714 BAC29178
GB AAH07541 AAH53436 AAL16953 AIC52640 EAW67531
REF NP_001178721 NP_001193057 NP_001248215 NP_116262 NP_789830
SP Q8BGG7 Q8TF42
TPG DAA22276