BMRB Entry 15211
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15211
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Title: Solution NMR structure of protein , Northeastprotein yxeF Structural Genomics Consortium target Sr500a
Deposition date: 2007-04-10 Original release date: 2007-06-05
Authors: Wu, Yibing; Liu, Gaohua; Zhang, Qi; Bhatnagar, Sagar; Chen, Chen X.; Nwosu, Chioma; Xiao, Rong; Cunningham, Kellie; Locke, Jessica; Ma, Li-Chung; Swapna, G.V.T; Baran, Michael C.; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas
Citation: Wu, Yibing; Liu, Gaohua; Zhang, Qi; Bhatnagar, Sagar; Chen, Chen X.; Nwosu, Chioma; Xiao, Rong; Cunningham, Kellie; Locke, Jessica; Ma, Li-Chung; Swapna, G.V.T; Baran, Michael C.; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of protein, Northeastprotein yxeF Structural Genomics Consortium target Sr500a" Protein Sci. ., .-..
Assembly members:
protein yxeF, polymer, 135 residues, 15450.332 Da.
Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: Bacillus Genus/species: subtilis subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
protein yxeF: MIMVSGCQQQKEETPFYYGT
WDEGRAPGPTDGVKSATVTF
TEDEVVETEVMEGRGEVQLP
FMAYKVISQSTDGSIEIQYL
GPYYPLKSTLKRGENGTLIW
EQNGQRKTMTRIESKTGREE
KDEKSKSLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 542 |
| 15N chemical shifts | 131 |
| 1H chemical shifts | 886 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein yxeF | 1 |
Entities:
Entity 1, protein yxeF 135 residues - 15450.332 Da.
| 1 | MET | ILE | MET | VAL | SER | GLY | CYS | GLN | GLN | GLN | ||||
| 2 | LYS | GLU | GLU | THR | PRO | PHE | TYR | TYR | GLY | THR | ||||
| 3 | TRP | ASP | GLU | GLY | ARG | ALA | PRO | GLY | PRO | THR | ||||
| 4 | ASP | GLY | VAL | LYS | SER | ALA | THR | VAL | THR | PHE | ||||
| 5 | THR | GLU | ASP | GLU | VAL | VAL | GLU | THR | GLU | VAL | ||||
| 6 | MET | GLU | GLY | ARG | GLY | GLU | VAL | GLN | LEU | PRO | ||||
| 7 | PHE | MET | ALA | TYR | LYS | VAL | ILE | SER | GLN | SER | ||||
| 8 | THR | ASP | GLY | SER | ILE | GLU | ILE | GLN | TYR | LEU | ||||
| 9 | GLY | PRO | TYR | TYR | PRO | LEU | LYS | SER | THR | LEU | ||||
| 10 | LYS | ARG | GLY | GLU | ASN | GLY | THR | LEU | ILE | TRP | ||||
| 11 | GLU | GLN | ASN | GLY | GLN | ARG | LYS | THR | MET | THR | ||||
| 12 | ARG | ILE | GLU | SER | LYS | THR | GLY | ARG | GLU | GLU | ||||
| 13 | LYS | ASP | GLU | LYS | SER | LYS | SER | LEU | GLU | HIS | ||||
| 14 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 1 mM; Tris 10 mM; NaCl 100%; NaN3 0.02%
sample_2: entity, [U-10% 13C; U-100% 15N], 1 mM; Tris 10 mM; NaCl 100%; NaN3 0.02%
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4,3D, GFT HNNCABCA | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT CABCACONNH | sample_1 | isotropic | sample_conditions_1 |
| 4,3D, GFT HCCH COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D, 15N-13C RESOLVED SIMULTANIOUS NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe v9.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
AutoStruct v1.0, Huang, Swapana, Rajan, Ke, Xia, Shukla, Inouye and Montelione - structure solution
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
XEASY, Bartels et al. - data analysis, peak picking
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA08322 BAI87653 BAM56043 BAM60055 GAK81370 |
| EMBL | CAB15993 CEI59768 CEJ79625 CJR72173 CON90429 |
| GB | ADV94782 AEP93027 AFQ59810 AGA22603 AGG63371 |
| REF | NP_391836 WP_003227095 WP_003244165 WP_014478470 WP_014481402 |
| SP | P54945 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts