BMRB Entry 15500
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15500
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Title: Solution Structure of the EDA-ID-related C417F mutant of human NEMO zinc finger PubMed: 18313693
Deposition date: 2007-09-28 Original release date: 2008-03-13
Authors: Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice
Citation: Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice. "Solution Structure of NEMO Zinc Finger and Impact of an Anhidrotic Ectodermal Dysplasia with Immunodeficiency-related Point Mutation" J. Mol. Biol. 377, 1419-1432 (2008).
Assembly members:
ZN, non-polymer, 65.409 Da.
NEMO_ZF-C417F, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NEMO_ZF-C417F: SSDFCCPKCQYQAPDMDTLQ
IHVMEFIE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 179 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ZINC ION | 1 |
| 2 | NEMO ZF-C417F | 2 |
Entities:
Entity 1, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Entity 2, NEMO ZF-C417F 28 residues - Formula weight is not available
Residues 3-28 corresponds to residues 394-419 of human NEMO zinc finger. Two Pro in the original sequence are replaced by two Ser (residues 1-2). Cys26 (i.e. C417 in human NEMO numbering) is mutated to Phe.
| 1 | SER | SER | ASP | PHE | CYS | CYS | PRO | LYS | CYS | GLN | ||||
| 2 | TYR | GLN | ALA | PRO | ASP | MET | ASP | THR | LEU | GLN | ||||
| 3 | ILE | HIS | VAL | MET | GLU | PHE | ILE | GLU |
Samples:
sample_1: NEMO ZF-C417F 1 mM; ZnS04 2 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D purged-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.03, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
ARIA v1.2, Linge, O, . - refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |