BMRB Entry 15943
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15943
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Title: Solution NMR Structure of protein of unknown function yegP from E. coli. Ontario Center for Structural Proteomics target EC0640_1_123 Northeast Structural Genomics Consortium Target ET102.
Deposition date: 2008-09-08 Original release date: 2009-04-23
Authors: Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Karra, Murthy; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl
Citation: Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Karra, Murthy; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl. "Solution structure of protein yegP from Escherichia Coli." Not known ., .-..
Assembly members:
protein_of_unkown_function_yegP_from_E.coli, polymer, 116 residues, 12566.956 Da.
Natural source: Common Name: not available Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
protein_of_unkown_function_yegP_from_E.coli: GVIMAGWFELSKSSDNQFRF
VLKAGNGETILTSELYTSKT
SAEKGIASVRSNSPQEERYE
KKTASNGKFYFNLKAANHQI
IGSSQMYATAQSRETGIASV
KANGTSQTVKDNTGSN
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 440 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 710 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 116 residues - 12566.956 Da.
| 1 | GLY | VAL | ILE | MET | ALA | GLY | TRP | PHE | GLU | LEU | ||||
| 2 | SER | LYS | SER | SER | ASP | ASN | GLN | PHE | ARG | PHE | ||||
| 3 | VAL | LEU | LYS | ALA | GLY | ASN | GLY | GLU | THR | ILE | ||||
| 4 | LEU | THR | SER | GLU | LEU | TYR | THR | SER | LYS | THR | ||||
| 5 | SER | ALA | GLU | LYS | GLY | ILE | ALA | SER | VAL | ARG | ||||
| 6 | SER | ASN | SER | PRO | GLN | GLU | GLU | ARG | TYR | GLU | ||||
| 7 | LYS | LYS | THR | ALA | SER | ASN | GLY | LYS | PHE | TYR | ||||
| 8 | PHE | ASN | LEU | LYS | ALA | ALA | ASN | HIS | GLN | ILE | ||||
| 9 | ILE | GLY | SER | SER | GLN | MET | TYR | ALA | THR | ALA | ||||
| 10 | GLN | SER | ARG | GLU | THR | GLY | ILE | ALA | SER | VAL | ||||
| 11 | LYS | ALA | ASN | GLY | THR | SER | GLN | THR | VAL | LYS | ||||
| 12 | ASP | ASN | THR | GLY | SER | ASN |
Samples:
sample_1: EC0640, [U-100% 13C; U-100% 15N], 0.4-0.7 mM; tris 10 mM; NaCl 300 mM; benzamidine 1 mM; NaN3 0.01%
sample_conditions_1: ionic strength: 300 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
ABACUS, Lemak, Steren, Arrowsmith, Llinas - chemical shift assignment, structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, BRUNGER, ADAMS, CLORE, GROS, NILGES AND READ - refinement
TALOS, Cornilescu, Delaglio and Bax - data analysis
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB36311 BAE76581 BAG77877 BAH46954 BAI26211 |
| EMBL | CAP76586 CAQ32494 CAQ89670 CAQ99003 CAR03465 |
| GB | AAC75141 AAG57143 AAN43679 AAN81062 AAP17505 |
| REF | NP_310915 NP_416584 NP_707972 WP_000899178 WP_000929408 |
| SP | P76402 Q83KI3 Q8FG00 Q8X7I0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts