BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16958

Title: 1H, 13C and 15N chemical shift assignments for ZCCHC9   PubMed: 20711761

Authors: Stefl, Richard; Sanudo, Maria; Jacko, Martin; Vanacova, Stepanka

Citation: Sanudo, Maria; Jacko, Martin; Rammelt, Christiane; Vanacova, Stepanka; Stefl, Richard. "(1)H, (13)C, and (15)N chemical shift assignments of ZCCHC9."  Biomol. NMR Assignments 5, 19-21 (2011).

Assembly members:
ZCCHC9, polymer, 164 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ZCCHC9: MQYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTEGSNA MVCFHCRKPGHGIADCPAAL ENQDMGTGICYRCGSTEHEI TKCKAKVDPALGEFPFAKCF VCGEMGHLSRSCPDNPKGLY ADGGGCKLCGSVEHLKKDCP ESQN

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts152
1H chemical shifts809

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZCCHC91
2Zn ion2

Entities:

Entity 1, ZCCHC9 164 residues - Formula weight is not available

1   METGLNTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLUGLYSERASNALA
7   METVALCYSPHEHISCYSARGLYSPROGLY
8   HISGLYILEALAASPCYSPROALAALALEU
9   GLUASNGLNASPMETGLYTHRGLYILECYS
10   TYRARGCYSGLYSERTHRGLUHISGLUILE
11   THRLYSCYSLYSALALYSVALASPPROALA
12   LEUGLYGLUPHEPROPHEALALYSCYSPHE
13   VALCYSGLYGLUMETGLYHISLEUSERARG
14   SERCYSPROASPASNPROLYSGLYLEUTYR
15   ALAASPGLYGLYGLYCYSLYSLEUCYSGLY
16   SERVALGLUHISLEULYSLYSASPCYSPRO
17   GLUSERGLNASN

Entity 2, Zn ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZCCHC9, [U-99% 13C; U-99% 15N], 2 mM; H2O 90%; D2O 10%; Tris buffer 20 mM; NaCl 200 mM; ZnSO4 100 %u03BCm; 2-mercaptoethanol 14 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAG36844
EMBL CAC21654 CAH92203
GB AAH32736 AIC57311 AIC60924 EAW95870 EAW95871
REF NP_001124507 NP_001124508 NP_001127522 NP_001182668 NP_115656
SP Q8N567