BMRB Entry 17614

Assembly:

Entities:

Entity 1, UCP2 303 residues - 32227.426 Da.

Residues 1-12 of the native sequence have been removed for structure determination

Entity 2, UCP2-SL1 303 residues - 32227.426 Da.

All native Cys have been mutated with C25A, C191S, C217S, C227S, C256S mutations. A single Cys (T68C) was introduced for MTSL labeling.

Entity 3, UCP2-SL2 303 residues - 32227.426 Da.

All native Cys have been mutated with C25A, C191S, C217S, C227S, C256S mutations. A single Cys (Q105C) was introduced for MTSL labeling.

Entity 4, UCP2-SL3 303 residues - 32227.426 Da.

All native Cys have been mutated with C25A, C191S, C217S, C227S, C256S mutations. A single Cys (D202C) was introduced for MTSL labeling.

Entity 5, UCP2-SL4 303 residues - 32227.426 Da.

All native Cys have been mutated with C25A, C191S, C217S, C227S, C256S mutations. A single Cys (H255C) was introduced for MTSL labeling.

Samples:

sample_1: UCP2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_2: UCP2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; DNA nanotube 2 ± 0.2 w/v; H2O 95%; D20 5%

sample_3: UCP2-SL1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_4: UCP2-SL2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_5: UCP2-SL3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_6: UCP2-SL4, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; guanosine diphosphate 5 mM; dodecylphosphocholine 150 mM; cardiolipin 1 mM; dimyristoyl-phosphatidylcholine 2 mM; beta-mercatpoethanol 5 mM; potassium phosphate 30 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 306 K

Experiments:

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

XEASY, Bartels et al. - chemical shift assignment

X-PLOR NIH v2.26, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

• Bruker Avance 600 MHz