BMRB Entry 17690
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17690
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Title: N-terminal regulatory segment of carnitine palmitoyltransferase 1A PubMed: 21990363
Deposition date: 2011-06-06 Original release date: 2011-10-26
Authors: Ulmer, Tobias; Rao, Jampani
Citation: Rao, Jampani; Warren, Gemma; Estolt-Povedano, Sara; Zammit, Victor; Ulmer, Tobias. "An environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A." J. Biol. Chem. 286, 42545-42554 (2011).
Assembly members:
carnitine palmitoyltransferase 1A, polymer, 42 residues, 4802.562 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
carnitine palmitoyltransferase 1A: MAEAHQAVAFQFTVTPDGID
LRLSHEALRQIYLSGLHSWK
KK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 112 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | carnitine palmitoyltransferase 1A | 1 |
Entities:
Entity 1, carnitine palmitoyltransferase 1A 42 residues - 4802.562 Da.
| 1 | MET | ALA | GLU | ALA | HIS | GLN | ALA | VAL | ALA | PHE | ||||
| 2 | GLN | PHE | THR | VAL | THR | PRO | ASP | GLY | ILE | ASP | ||||
| 3 | LEU | ARG | LEU | SER | HIS | GLU | ALA | LEU | ARG | GLN | ||||
| 4 | ILE | TYR | LEU | SER | GLY | LEU | HIS | SER | TRP | LYS | ||||
| 5 | LYS | LYS |
Samples:
sample_1: palmitoyltransferase_1A, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; palmitoyltransferase_1A, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 95%; D2O 5%; MES+NaOH 25 mM; dodecytrimethylammonium chloride 150 mM
sample_conditions_1: ionic strength: 0.025 M; pH: 5.6; pressure: 1 atm; temperature: 308.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| quant j correlation | sample_1 | isotropic | sample_conditions_1 |
| 3D NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAG36955 |
| EMBL | CAC80073 CAD12625 CAD59673 |
| GB | AAC41748 AAH00185 AAK69171 AAP88793 AAX32217 |
| PRF | 2108402A |
| REF | NP_001009414 NP_001027017 NP_001123277 NP_001273789 NP_001291918 |
| SP | P50416 |
| TPG | DAA13488 |
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