BMRB Entry 18110
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18110
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Title: Solution Structure of C-terminal RAGE (ctRAGE) PubMed: 2194616
Deposition date: 2011-11-29 Original release date: 2011-12-20
Authors: Rai, Vivek; Maldonado, Andres; Burz, David; Reverdatto, Sergey; Schmidt, Ann Marie; Shekhtman, Alexander
Citation: Sinclair, A.; Barnett, A.; Lunec, J.. "Free radicals and antioxidant systems in health and disease." Br. J. Hosp. Med. 43, 334-344 (1990).
Assembly members:
ctRAGE, polymer, 43 residues, 1990.314 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ctRAGE: MWQRRQRRGEERKAPENQEE
EEERAELNQSEEPEAGESST
GGP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 107 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C-terminal RAGE (ctRAGE) | 1 |
Entities:
Entity 1, C-terminal RAGE (ctRAGE) 43 residues - 1990.314 Da.
| 1 | MET | TRP | GLN | ARG | ARG | GLN | ARG | ARG | GLY | GLU | ||||
| 2 | GLU | ARG | LYS | ALA | PRO | GLU | ASN | GLN | GLU | GLU | ||||
| 3 | GLU | GLU | GLU | ARG | ALA | GLU | LEU | ASN | GLN | SER | ||||
| 4 | GLU | GLU | PRO | GLU | ALA | GLY | GLU | SER | SER | THR | ||||
| 5 | GLY | GLY | PRO |
Samples:
sample_1: ctRAGE, [U-99% 13C; U-99% 15N], 300 uM; D2O, [U-100% 2H], 10%; potassium phosphate 10 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%
sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, G??ntert P. - refinement
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| DBJ | BAA05958 BAA89369 BAC65466 BAG35995 BAG60385 |
| EMBL | CAB43094 |
| GB | AAA03574 AAB47491 AAH20669 AAX07272 AAX07274 |
| REF | NP_001127 NP_001193858 NP_001193861 |
| SP | Q15109 |
Download simulated HSQC data in one of the following formats:
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