BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18175

Title: 40-residue D23N beta amyloid fibril   PubMed: 22403062

Deposition date: 2012-01-03 Original release date: 2012-02-06

Authors: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert

Citation: Qiang, Wei; Yau, Wai-Ming; Luo, Yongquan; Mattson, Mark; Tycko, Robert. "Antiparallel -sheet architecture in Iowa-mutant -amyloid fibrils."  Proc. Natl. Acad. Sci. U.S.A. 109, 4443-4448 (2012).

Assembly members:
entity, polymer, 26 residues, 2650.167 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: QKLVFFAENVGSNKGAIIGL MVGGVV

Data sets:
Data typeCount
13C chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1D23N beta amyloid fibril, chain 11
2D23N beta amyloid fibril, chain 21
3D23N beta amyloid fibril, chain 31
4D23N beta amyloid fibril, chain 41
5D23N beta amyloid fibril, chain 51
6D23N beta amyloid fibril, chain 61
7D23N beta amyloid fibril, chain 71
8D23N beta amyloid fibril, chain 81

Entities:

Entity 1, D23N beta amyloid fibril, chain 1 26 residues - 2650.167 Da.

1   GLNLYSLEUVALPHEPHEALAGLUASNVAL
2   GLYSERASNLYSGLYALAILEILEGLYLEU
3   METVALGLYGLYVALVAL

Samples:

sample_1: entity, [U-13C], 25 uM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C fpRFDRsample_1solidsample_conditions_1
2D 13C-13C RADsample_1solidsample_conditions_1
2D 13C-13C CHHCsample_1solidsample_conditions_1
1D 13C PITHIRDs-CTsample_1solidsample_conditions_1

Software:

X-PLOR NIH v2.28.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian InfinityPlus 400 MHz

Related Database Links:

BMRB 17774 25004
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