BMRB Entry 18809
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18809
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Title: SP-B C-terminal (residues 59-80) peptide in methanol
Deposition date: 2012-10-25 Original release date: 2014-04-21
Authors: Kuznetsova, Anna; Long, Joanna
Citation: Kuznetsova, Anna; Vanni, Julieann; Long, Joanna. "Solution NMR structures of the C-terminal segment of surfactant protein B (residues 59-80) in DPC detergent micelles and methanol." Biochemistry ., .-..
Assembly members:
SP-B_C-terminal, polymer, 22 residues, 2536.138 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SP-B_C-terminal: DTLLGRMLPQLVCRLVLRCS
MD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 167 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SP-B C-terminal residues 59-80 | 1 |
Entities:
Entity 1, SP-B C-terminal residues 59-80 22 residues - 2536.138 Da.
| 1 | ASP | THR | LEU | LEU | GLY | ARG | MET | LEU | PRO | GLN | ||||
| 2 | LEU | VAL | CYS | ARG | LEU | VAL | LEU | ARG | CYS | SER | ||||
| 3 | MET | ASP |
Samples:
SP-B_residues_59-80_in_methanol: SP-B_C-terminal 1.2 mM; TCEP 20 mM
300K: ionic strength: 20 mM; pH: 4; pressure: 1 atm; temperature controller setting: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | SP-B_residues_59-80_in_methanol | isotropic | 300K |
| 2D 1H-1H NOESY | SP-B_residues_59-80_in_methanol | isotropic | 300K |
Software:
ProcheckNMR, Laskowski and MacArthur - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG37452 BAG64767 |
| GB | AAA36628 AAA60212 AAA88099 AAB59541 AAH32785 |
| REF | NP_000533 NP_942140 XP_003816622 XP_003816624 XP_005264544 |
| SP | P07988 |