BMRB Entry 19406
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19406
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Title: K11-linked Diubiquitin average solution structure at pH 6.8, 0 mM NaCl PubMed: 23823328
Deposition date: 2013-08-02 Original release date: 2013-09-03
Authors: Castaneda, Carlos; Fushman, David
Citation: Castaneda, Carlos; Kashyap, Tanuja; Nakasone, Mark; Krueger, Susan; Fushman, David. "Unique structural, dynamical, and functional properties of K11-linked polyubiquitin chains" Structure 21, 1168-1181 (2013).
Assembly members:
entity, polymer, 152 residues, 8576.914 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGGMQIF
VKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKE
STLHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 140 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | K11-linked Diubiquitin_1 | 1 |
| 2 | K11-linked Diubiquitin_2 | 1 |
Entities:
Entity 1, K11-linked Diubiquitin_1 152 residues - 8576.914 Da.
| 1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY | MET | GLN | ILE | PHE | ||||
| 9 | VAL | LYS | THR | LEU | THR | GLY | LYS | THR | ILE | THR | ||||
| 10 | LEU | GLU | VAL | GLU | PRO | SER | ASP | THR | ILE | GLU | ||||
| 11 | ASN | VAL | LYS | ALA | LYS | ILE | GLN | ASP | LYS | GLU | ||||
| 12 | GLY | ILE | PRO | PRO | ASP | GLN | GLN | ARG | LEU | ILE | ||||
| 13 | PHE | ALA | GLY | LYS | GLN | LEU | GLU | ASP | GLY | ARG | ||||
| 14 | THR | LEU | SER | ASP | TYR | ASN | ILE | GLN | LYS | GLU | ||||
| 15 | SER | THR | LEU | HIS | LEU | VAL | LEU | ARG | LEU | ARG | ||||
| 16 | GLY | GLY |
Samples:
sample_1: entity, [U-99% 15N], 75 uM; sodium phosphate 20 mM; H2O 93%; D2O 7%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N IPAP HSQC | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - collection, data analysis, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 11505 11547 15047 15410 15907 16228 16582 16626 17181 17439 17769 17919 18582 18583 18584 18610 18611 18737 19412 25070 25601 26604 4245 4375 |
| PDB | |
| DBJ | BAA03983 BAA09096 BAA09860 BAA11842 BAA11843 |
| EMBL | CAA25706 CAA26488 CAA28495 CAA30183 CAA30815 |
| GB | AAA28154 AAA28997 AAA28998 AAA28999 AAA29000 |
| PIR | I50437 I51568 I65237 JN0790 JT0492 |
| PRF | 1101405A 1212243A 1212243C 1212243F 1212243G |
| REF | NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286 |
| SP | P0C273 P0C275 P0C276 P0CG47 P0CG48 |
| TPD | FAA00319 |
| TPE | CEL68433 CEL70397 CEL75964 CEL78064 |
| TPG | DAA18802 DAA20663 DAA20672 DAA24675 DAA28295 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts