BMRB Entry 25131
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25131
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Title: 1H chemical shift assignment of Kindlin-2 F2
Deposition date: 2014-08-05 Original release date: 2014-08-25
Authors: Fukuda, Koichi; Bledzka, Kamila; Yang, Jun; Perera, H.
Citation: Fukuda, Koichi; Bledzka, Kamila; Yang, Jun; Perera, H.; Edward, Plow; Qin, Jun. "kindlin-2 F2 domain" J. Biol. Chem. ., .-..
Assembly members:
entity, polymer, 20 residues, 2192.330 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: SDKEVDEVDAALSDLEITLE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kindlin-2 F2 | 1 |
Entities:
Entity 1, Kindlin-2 F2 20 residues - 2192.330 Da.
| 1 | SER | ASP | LYS | GLU | VAL | ASP | GLU | VAL | ASP | ALA | |
| 2 | ALA | LEU | SER | ASP | LEU | GLU | ILE | THR | LEU | GLU |
Samples:
sample_1: HEPES 20 mM; sodium chloride 100 mM; DTT 2 mM; K2F2 peptide 2 mM; ILK KLD/a-parvin CH2 50 uM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 273 K; pH: 6.8; pressure: 1 atm; ionic strength: 100 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
XPLOR-NIH v2.25, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAE26194 BAE38850 BAF84427 |
| EMBL | CAA80852 CAD61925 |
| GB | AAH17327 AAH33436 AAH77819 AAH83876 AAI51293 |
| REF | NP_001011915 NP_001086955 NP_001094734 NP_001128471 NP_001128472 |
| SP | Q8CIB5 Q96AC1 |
| TPG | DAA25234 |