BMRB Entry 25541
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25541
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Title: Solution structure of MyUb (1080-1122) of human Myosin VI PubMed: 26971995
Deposition date: 2015-03-20 Original release date: 2016-03-07
Authors: He, Fahu; Walters, Kylie
Citation: He, Fahu; Wollscheid, Hans-Peter; Nowicka, Urszula; Biancospino, Matteo; Valentini, Eleonora; Ehlinger, Aaron; Acconcia, Filippo; Magistrati, Elisa; Polo, Simona; Walters, Kylie. "Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains" Cell Rep. 14, 2683-2694 (2016).
Assembly members:
entity, polymer, 51 residues, 5213.026 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GPLGSPNSGTKKYDLSKWKY
AELRDTINTSCDIELLAACR
EEFHRRLKVYH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 232 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 364 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 51 residues - 5213.026 Da.
The first 8 residues (GPLGSPNS) are a tag
| 1 | GLY | PRO | LEU | GLY | SER | PRO | ASN | SER | GLY | THR | ||||
| 2 | LYS | LYS | TYR | ASP | LEU | SER | LYS | TRP | LYS | TYR | ||||
| 3 | ALA | GLU | LEU | ARG | ASP | THR | ILE | ASN | THR | SER | ||||
| 4 | CYS | ASP | ILE | GLU | LEU | LEU | ALA | ALA | CYS | ARG | ||||
| 5 | GLU | GLU | PHE | HIS | ARG | ARG | LEU | LYS | VAL | TYR | ||||
| 6 | HIS |
Samples:
sample_1: entity, [U-13C; U-15N], 0.3 mM; sodium phosphate buffer 20 mM; NaCl 50 mM; DTT 2 mM; NaN3 0.1%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Kujira, Naohiro Kobayashi - chemical shift assignment
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker Avance 700 MHz
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