BMRB Entry 25560
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25560
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Title: Solution structure of the GBII-beta MRH domain W409A point mutant
Deposition date: 2015-04-01 Original release date: 2015-12-07
Authors: Olson, Linda; Peterson, Francis; Damhs, Nancy
Citation: Olson, Linda; Orsi, Ramiro; Peterson, Francis; Parodi, Armando; Kim, Jung-Ja; D'Alessio, Cecilia; Damhs, Nancy. "Crystal structure and functional analyses of glucosdidase II's lectin domain: Insgihts into oligomannose recognition" Not known ., .-..
Assembly members:
entity, polymer, 94 residues, 10428.918 Da.
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: YRAIKGMETKREIGGYTYKV
VFYENVFQDSILLGNFASQE
GNVLKYENGQSCANGPHRSA
IVTVECGVENEIVSVLEAQK
CEYLIKMKSPAACS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 619 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 94 residues - 10428.918 Da.
| 1 | TYR | ARG | ALA | ILE | LYS | GLY | MET | GLU | THR | LYS | ||||
| 2 | ARG | GLU | ILE | GLY | GLY | TYR | THR | TYR | LYS | VAL | ||||
| 3 | VAL | PHE | TYR | GLU | ASN | VAL | PHE | GLN | ASP | SER | ||||
| 4 | ILE | LEU | LEU | GLY | ASN | PHE | ALA | SER | GLN | GLU | ||||
| 5 | GLY | ASN | VAL | LEU | LYS | TYR | GLU | ASN | GLY | GLN | ||||
| 6 | SER | CYS | ALA | ASN | GLY | PRO | HIS | ARG | SER | ALA | ||||
| 7 | ILE | VAL | THR | VAL | GLU | CYS | GLY | VAL | GLU | ASN | ||||
| 8 | GLU | ILE | VAL | SER | VAL | LEU | GLU | ALA | GLN | LYS | ||||
| 9 | CYS | GLU | TYR | LEU | ILE | LYS | MET | LYS | SER | PRO | ||||
| 10 | ALA | ALA | CYS | SER |
Samples:
sample_1: MRH GIIb, [U-99% 13C; U-99% 15N], 0.7 mM; imidazole, [U-99% 2H], 10 mM; sodium chloride 150 mM; sodium azide 0.02%; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.150 M; pH: 7.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guentert P. - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
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SPARKY: Backbone
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