BMRB Entry 25905
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25905
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Title: Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)
Deposition date: 2015-11-23 Original release date: 2016-11-17
Authors: Yang, Xiao
Citation: Yang, Xiao. "Solution structure of the bromodomain of Trypanosoma brucei Bromodomain Factor 2(BDF2)" Not known ., .-..
Assembly members:
entity, polymer, 111 residues, 12723.368 Da.
Natural source: Common Name: kinetoplastids Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MSKNERDTSFNKNGCLVFVS
RLWDLDKLGMFHHPVSAEEL
PDYHTVIKRPVDLSSIRDGI
EKGTYATDVDVQNDVARMIT
NALEYNAKGSTWYQEAMSFR
KTYLDLARQSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 292 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 501 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 111 residues - 12723.368 Da.
| 1 | MET | SER | LYS | ASN | GLU | ARG | ASP | THR | SER | PHE | ||||
| 2 | ASN | LYS | ASN | GLY | CYS | LEU | VAL | PHE | VAL | SER | ||||
| 3 | ARG | LEU | TRP | ASP | LEU | ASP | LYS | LEU | GLY | MET | ||||
| 4 | PHE | HIS | HIS | PRO | VAL | SER | ALA | GLU | GLU | LEU | ||||
| 5 | PRO | ASP | TYR | HIS | THR | VAL | ILE | LYS | ARG | PRO | ||||
| 6 | VAL | ASP | LEU | SER | SER | ILE | ARG | ASP | GLY | ILE | ||||
| 7 | GLU | LYS | GLY | THR | TYR | ALA | THR | ASP | VAL | ASP | ||||
| 8 | VAL | GLN | ASN | ASP | VAL | ALA | ARG | MET | ILE | THR | ||||
| 9 | ASN | ALA | LEU | GLU | TYR | ASN | ALA | LYS | GLY | SER | ||||
| 10 | THR | TRP | TYR | GLN | GLU | ALA | MET | SER | PHE | ARG | ||||
| 11 | LYS | THR | TYR | LEU | ASP | LEU | ALA | ARG | GLN | SER | ||||
| 12 | GLY |
Samples:
sample_1: entity 0.5 mM; sodium chloride 150 mM; sodium phosphate 20 mM; DTT 1 mM; EDTA 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker DMX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts