BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26890

Title: Resonance assignments of an alpha-synuclein fibril prepared in Tris buffer at moderate ionic strength   PubMed: 29476328

Deposition date: 2016-09-08 Original release date: 2018-02-28

Authors: Barclay, Alexander; Dhavale, Dhruva; Courtney, Joseph; Kotzbauer, Paul; Rienstra, Chad

Citation: Barclay, Alexander; Dhavale, Dhruva; Courtney, Joseph; Kotzbauer, Paul; Rienstra, Chad. "Resonance assignments of an alpha-synuclein fibril prepared in Tris buffer at moderate ionic strength"  Biomol. NMR Assign. 12, 195-199 (2018).

Assembly members:
alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha-synuclein: MDVFMKGKSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: alpha-synuclein, [U-13C; U-15N], 17 mg

sample_conditions_1: pH: 8.0; temperature: 263 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_1
2D NCA(CX)sample_1isotropicsample_conditions_1
3D NCACX DARRsample_1isotropicsample_conditions_1
3D NCOCX DARRsample_1isotropicsample_conditions_1
2D CA(NCO)CX DARRsample_1isotropicsample_conditions_1
3D CANCO(CX)sample_1isotropicsample_conditions_1
3D CAN(CO)CX DARRsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian Infinity Plus 600 MHz