BMRB Entry 27034
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27034
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Title: 1H, 13C and 15N chemical shift assignments of A. thaliana RCD1(468-569) PubMed: 28593560
Deposition date: 2017-02-13 Original release date: 2017-06-13
Authors: Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu
Citation: Tossavainen, Helena; Hellman, Maarit; Vainonen, Julia; Kangasjarvi, Jaakko; Permi, Perttu. "1H, 13C and 15N NMR chemical shift assignments of A. thaliana RCD1 RST" Biomol. NMR Assignments 11, 207-210 (2017).
Assembly members:
RCD1, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RCD1: GSPEFMGVTLEGPKDLPPQL
ESNQGARGSGSANSVGSSTT
RPKSPWMPFPTLFAAISHKV
AENDMLLINADYQQLRDKKM
TRAEFVRKLRVIVGDDLLRS
TITTLQNQPKSKEIPGSIRD
HEEGAGGL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 500 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 811 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RCD1 | 1 |
Entities:
Entity 1, RCD1 128 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLU | PHE | MET | GLY | VAL | THR | LEU | ||||
| 2 | GLU | GLY | PRO | LYS | ASP | LEU | PRO | PRO | GLN | LEU | ||||
| 3 | GLU | SER | ASN | GLN | GLY | ALA | ARG | GLY | SER | GLY | ||||
| 4 | SER | ALA | ASN | SER | VAL | GLY | SER | SER | THR | THR | ||||
| 5 | ARG | PRO | LYS | SER | PRO | TRP | MET | PRO | PHE | PRO | ||||
| 6 | THR | LEU | PHE | ALA | ALA | ILE | SER | HIS | LYS | VAL | ||||
| 7 | ALA | GLU | ASN | ASP | MET | LEU | LEU | ILE | ASN | ALA | ||||
| 8 | ASP | TYR | GLN | GLN | LEU | ARG | ASP | LYS | LYS | MET | ||||
| 9 | THR | ARG | ALA | GLU | PHE | VAL | ARG | LYS | LEU | ARG | ||||
| 10 | VAL | ILE | VAL | GLY | ASP | ASP | LEU | LEU | ARG | SER | ||||
| 11 | THR | ILE | THR | THR | LEU | GLN | ASN | GLN | PRO | LYS | ||||
| 12 | SER | LYS | GLU | ILE | PRO | GLY | SER | ILE | ARG | ASP | ||||
| 13 | HIS | GLU | GLU | GLY | ALA | GLY | GLY | LEU |
Samples:
sample_1: RCD1, [U-13C; U-15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D iHNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D hCCmHm | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts