BMRB Entry 27039
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27039
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Title: Chemical shifts of amorphous-looking aggregates of the P23T mutant of human gamma-D-crystallin formed at pH 7. PubMed: 28474685
Deposition date: 2017-02-26 Original release date: 2017-05-02
Authors: Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick
Citation: Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick. "Cataract-associated P23T gamma-D-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH" Nat Commun 8, 15137-15137 (2017).
Assembly members:
gD_crystallin_P23T, polymer, 173 residues, 20616.9178 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
gD_crystallin_P23T: GKITLYEDRGFQGRHYECSS
DHTNLQPYLSRCNSARVDSG
CWMLYEQPNYSGLQYFLRRG
DYADHQQWMGLSDSVRSCRL
IPHSGSHRIRLYEREDYRGQ
MIEFTEDCSCLQDRFRFNEI
HSLNVLEGSWVLYELSNYRG
RQYLLMPGDYRRYQDWGATN
ARVGSLRRVIDFS
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 157 |
15N chemical shifts | 39 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | gDcrystallinP23T | 1 |
Entities:
Entity 1, gDcrystallinP23T 173 residues - 20616.9178 Da.
1 | GLY | LYS | ILE | THR | LEU | TYR | GLU | ASP | ARG | GLY | ||||
2 | PHE | GLN | GLY | ARG | HIS | TYR | GLU | CYS | SER | SER | ||||
3 | ASP | HIS | THR | ASN | LEU | GLN | PRO | TYR | LEU | SER | ||||
4 | ARG | CYS | ASN | SER | ALA | ARG | VAL | ASP | SER | GLY | ||||
5 | CYS | TRP | MET | LEU | TYR | GLU | GLN | PRO | ASN | TYR | ||||
6 | SER | GLY | LEU | GLN | TYR | PHE | LEU | ARG | ARG | GLY | ||||
7 | ASP | TYR | ALA | ASP | HIS | GLN | GLN | TRP | MET | GLY | ||||
8 | LEU | SER | ASP | SER | VAL | ARG | SER | CYS | ARG | LEU | ||||
9 | ILE | PRO | HIS | SER | GLY | SER | HIS | ARG | ILE | ARG | ||||
10 | LEU | TYR | GLU | ARG | GLU | ASP | TYR | ARG | GLY | GLN | ||||
11 | MET | ILE | GLU | PHE | THR | GLU | ASP | CYS | SER | CYS | ||||
12 | LEU | GLN | ASP | ARG | PHE | ARG | PHE | ASN | GLU | ILE | ||||
13 | HIS | SER | LEU | ASN | VAL | LEU | GLU | GLY | SER | TRP | ||||
14 | VAL | LEU | TYR | GLU | LEU | SER | ASN | TYR | ARG | GLY | ||||
15 | ARG | GLN | TYR | LEU | LEU | MET | PRO | GLY | ASP | TYR | ||||
16 | ARG | ARG | TYR | GLN | ASP | TRP | GLY | ALA | THR | ASN | ||||
17 | ALA | ARG | VAL | GLY | SER | LEU | ARG | ARG | VAL | ILE | ||||
18 | ASP | PHE | SER |
Samples:
gDcrytallinP23T: gD crystallin P23T, [U-99% 13C; U-99% 15N], mg
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D NCACX | gDcrytallinP23T | isotropic | sample_conditions_1 |
3D NCOCX | gDcrytallinP23T | isotropic | sample_conditions_1 |
2D DARR | gDcrytallinP23T | isotropic | sample_conditions_1 |
2D NCACX | gDcrytallinP23T | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz