BMRB Entry 27439
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27439
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Title: Sph15 PubMed: 30284185
Deposition date: 2018-03-30 Original release date: 2018-10-04
Authors: Coulthard, Rachel; Rajasekar, Karthik; Hyde, Eva; Smith, Lorna
Citation: Coulthard, Rachel; Rajasekar, Karthik; Hyde, Eva; Smith, Lorna. "1H, 13C and 15N NMR assignments of self-incompatibility protein homologue 15 from Arabidopsis thaliana" Biomol. NMR Assignments 13, 67-70 (2019).
Assembly members:
Sph15, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sph15: AMGCKEIEIVIKNTLGPSRI
LQYHCRSGNTNVGVQYLNFK
GTRIIKFKDDGTERSRWNCL
FRQGINMKFFTEVEAYRPDL
KHPLCGKRYELSARMDAIYF
KMDERPPQPLNKWRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 393 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 598 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sph15 | 1 |
Entities:
Entity 1, Sph15 115 residues - Formula weight is not available
| 1 | ALA | MET | GLY | CYS | LYS | GLU | ILE | GLU | ILE | VAL | ||||
| 2 | ILE | LYS | ASN | THR | LEU | GLY | PRO | SER | ARG | ILE | ||||
| 3 | LEU | GLN | TYR | HIS | CYS | ARG | SER | GLY | ASN | THR | ||||
| 4 | ASN | VAL | GLY | VAL | GLN | TYR | LEU | ASN | PHE | LYS | ||||
| 5 | GLY | THR | ARG | ILE | ILE | LYS | PHE | LYS | ASP | ASP | ||||
| 6 | GLY | THR | GLU | ARG | SER | ARG | TRP | ASN | CYS | LEU | ||||
| 7 | PHE | ARG | GLN | GLY | ILE | ASN | MET | LYS | PHE | PHE | ||||
| 8 | THR | GLU | VAL | GLU | ALA | TYR | ARG | PRO | ASP | LEU | ||||
| 9 | LYS | HIS | PRO | LEU | CYS | GLY | LYS | ARG | TYR | GLU | ||||
| 10 | LEU | SER | ALA | ARG | MET | ASP | ALA | ILE | TYR | PHE | ||||
| 11 | LYS | MET | ASP | GLU | ARG | PRO | PRO | GLN | PRO | LEU | ||||
| 12 | ASN | LYS | TRP | ARG | SER |
Samples:
sample_1: Sph15, [U-99% 13C; U-99% 15N], 0.3 mM; sodium chloride 50 mM
sample_2: Sph15, [U-99% 13C; U-99% 15N], 1 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 5.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPN vV2, CCPN - chemical shift assignment
ARIA v2.3, Linge, O'Donoghue and Nilges - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz
- Varian INOVA 800 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts