BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27457

Title: 13C, 15N solid-state NMR assignments of human histone H4 in nucleosome core particle   PubMed: 29905032

Authors: Shi, Xiangyan; Prasanna, Chinmayi; Pervushin, Konstantin; Nordenskiold, Lars

Citation: Shi, Xiangyan; Prasanna, Chinmayi; Nagashima, Toshio; Yamazaki, Toshio; Pervushin, Konstantin; Nordenskiold, Lars. "Structure and Dynamics in the Nucleosome Revealed by Solid-State NMR"  Angew. Chem. Int. Ed. Engl. 57, 9734-9738 (2018).

Assembly members:
hH4-nucleosome, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hH4-nucleosome: SGRGKGGKGLGKGGAKRHRK VLRDNIQGITKPAIRRLARR GGVKRISGLIYEETRGVLKV FLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGF GG

Data sets:
Data typeCount
13C chemical shifts294
15N chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hH41

Entities:

Entity 1, hH4 102 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Samples:

sample_1: hH4-nucleosome, [U-100% 13C; U-100% 15N], 2.8 mg; hH2A-nucleosome 3.2 mg; hH2B-nucleosome 3.2 mg; hH3-nucleosome 3.5 mg; 601 Widom DNA 10.3 mg

sample_conditions_1: ionic strength: 20 mM; pH: 7.5; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D CC DARR with 20 ms mixing timesample_1solidsample_conditions_1
2D CC DARR with 100 ms mixing timesample_1solidsample_conditions_1
2D NCAsample_1solidsample_conditions_1
2D NCOsample_1solidsample_conditions_1
3D NCACX with 100 ms CC DARRsample_1solidsample_conditions_1
3D NCOCX with 100 ms CC DARRsample_1solidsample_conditions_1
3D CANCOsample_1solidsample_conditions_1

Software:

SPARKY v3.116, Goddard - chemical shift assignment

TOPSPIN v3.5pl6, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

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