BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27468

Title: Isoleucine chemical shift assignment of gp17.1 based on the precursor 2-Ketobutyric acid-13C4-3,3-d2 by proton-detected solid-state NMR   PubMed: 29917302

Authors: Zinke, Maximilian; Lange, Adam

Citation: Zinke, Maximilian; Fricke, Pascal; Lange, Sascha; Zinn-Justin, Sophie; Lange, Adam. "Protein-Protein Interfaces Probed by Methyl Labeling and Proton-Detected Solid-State NMR Spectroscopy"  Chemphyschem 19, 2457-2460 (2018).

Assembly members:
gp17.1, polymer, 185 residues, Formula weight is not available

Natural source:   Common Name: SPP1 bacteriophage   Taxonomy ID: 38018   Superkingdom: Viruses   Kingdom: not available   Genus/species: SPP1 bacteriophage

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
gp17.1: MGMPETPIMGQDVKYLFQSI DAATGSAPLFPAYQTDGSVS GERELFDEQTKNGRILGPGS VADSGEVTYYGKRGDAGQKA IEDAYQNGKQIKFWRVDTVK NENDKYDAQFGFAYIESREY SDGVEGAVEISISLQVIGEL KNGEIDTLPEEIVNVSKGGY DFQQPGQTTGEAPGTVPAPH HHHHH

Data sets:
Data typeCount
13C chemical shifts30
15N chemical shifts10
1H chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1gp17.1 phage tail1

Entities:

Entity 1, gp17.1 phage tail 185 residues - Formula weight is not available

1   METGLYMETPROGLUTHRPROILEMETGLY
2   GLNASPVALLYSTYRLEUPHEGLNSERILE
3   ASPALAALATHRGLYSERALAPROLEUPHE
4   PROALATYRGLNTHRASPGLYSERVALSER
5   GLYGLUARGGLULEUPHEASPGLUGLNTHR
6   LYSASNGLYARGILELEUGLYPROGLYSER
7   VALALAASPSERGLYGLUVALTHRTYRTYR
8   GLYLYSARGGLYASPALAGLYGLNLYSALA
9   ILEGLUASPALATYRGLNASNGLYLYSGLN
10   ILELYSPHETRPARGVALASPTHRVALLYS
11   ASNGLUASNASPLYSTYRASPALAGLNPHE
12   GLYPHEALATYRILEGLUSERARGGLUTYR
13   SERASPGLYVALGLUGLYALAVALGLUILE
14   SERILESERLEUGLNVALILEGLYGLULEU
15   LYSASNGLYGLUILEASPTHRLEUPROGLU
16   GLUILEVALASNVALSERLYSGLYGLYTYR
17   ASPPHEGLNGLNPROGLYGLNTHRTHRGLY
18   GLUALAPROGLYTHRVALPROALAPROHIS
19   HISHISHISHISHIS

Samples:

sample_1: gp17.1, Isoleucine-(1Hdelta1, 13Cdelta1, 13Cgamma1, 13Calpha, 13CO)-u-15N; 100% back-exchange, 13 mg; sodium phosphate 20 mM; sodium chloride 500 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 7.4; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
2D HccanHsample_1isotropicsample_conditions_1
3D HNhHsample_1isotropicsample_conditions_1
2D hCHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

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