BMRB Entry 30490
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30490
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Title: Solution structure of Rbfox2 RRM mimetic peptide CPfox7 PubMed: 30537200
Deposition date: 2018-07-03 Original release date: 2019-05-07
Authors: Sun, Y.-T.; Varani, G.
Citation: Sun, Yi-Ting; Shortridge, Matthew; Varani, Gabriele. "A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b." Chembiochem 20, 931-939 (2019).
Assembly members:
entity_1, polymer, 16 residues, 2024.398 Da.
Natural source: Common Name: not available Taxonomy ID: 36630 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GSRRFRFXPKIIFNQR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 16 residues - 2024.398 Da.
| 1 | GLY | SER | ARG | ARG | PHE | ARG | PHE | DPR | PRO | LYS | ||||
| 2 | ILE | ILE | PHE | ASN | GLN | ARG |
Samples:
sample_1: CPfox7 3.0 mg/mL; potassium phosphate 10 mM; potassium chloride 10 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - processing
NMR spectrometers:
- Bruker AvanceIII 600 MHz