BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4174

Title: Chemical Shift Assignments of the Mg2+-Loaded State of the N-Terminal Domain of Calmodulin

Deposition date: 1998-08-11 Original release date: 2000-04-03

Authors: Malmendal, Anders; Evenas, Johan; Thulin, Eva; Gippert, Garry; Drakenberg, Torbjorn; Forsen, Sture

Citation: Malmendal, Anders; Evenas, Johan; Thulin, Eva; Gippert, Garry; Drakenberg, Torbjorn; Forsen, Sture. "When Size is Important: Accommodation of Magnesium in a Calcium Binding Regulatory Domain"  J. Biol. Chem. 273, 28994-29001 (1998).

Assembly members:
N-terminal domain of calmodulin, polymer, 81 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: .

Entity Sequences (FASTA):
N-terminal domain of calmodulin: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARKMKDT D

Data sets:
Data typeCount
15N chemical shifts84
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TR1C1
2Mg2

Entities:

Entity 1, TR1C 81 residues - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYTHRILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   THRMETMETALAARGLYSMETLYSASPTHR
9   ASP

Entity 2, Mg - Mg - 24.305 Da.

1   MG

Samples:

sample_one: N-terminal domain of calmodulin 4.0 mM; D2O 10%; H2O 90%; KCL 10 mM; Azide 100 uM

sample_two: N-terminal domain of calmodulin, [U-15N], 4.0 mM; D2O 10%; H2O 90%; KCL 10 mM; Azide 100 uM

sample_conditions_one: ionic strength: 0.1; pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 18082 18302
PDB
EMBL CAA29381 CAB65357 CCD82979 CUA69889
GB AAH53790 ABY16748 ABY16749 ABY16752 ACH46202
REF XP_001869424 XP_001995129 XP_002772218 XP_006032607 XP_006137175

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