BMRB Entry 4326
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4326
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Title: Complete 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Domain of DNA Polymerase B (residues 2-87).
Deposition date: 1999-03-25 Original release date: 1999-05-19
Authors: Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory
Citation: Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory. "Assignments of 1H, 15N, and 13C Resonances for the Backbone and Side Chains of the N-Terminal Domain of DNA Polymerase B. Determination of the Secondary Structure and Tertiary Contacts" Biochemistry 33, 9537-9545 (1994).
Assembly members:
DNA Polymerase B, polymer, 87 residues, 9490 Da.
Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DNA Polymerase B: MSKRKAPQETLNGGITDMLV
ELANFEKNVSQAIHKYNAYR
KAASVIAKYPHKIKSGAEAK
KLPGVGTKIAEKIDEFLATG
KLRKLEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 312 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 598 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | B-Pol N-terminal Domain | 1 |
Entities:
Entity 1, B-Pol N-terminal Domain 87 residues - 9490 Da.
| 1 | MET | SER | LYS | ARG | LYS | ALA | PRO | GLN | GLU | THR | ||||
| 2 | LEU | ASN | GLY | GLY | ILE | THR | ASP | MET | LEU | VAL | ||||
| 3 | GLU | LEU | ALA | ASN | PHE | GLU | LYS | ASN | VAL | SER | ||||
| 4 | GLN | ALA | ILE | HIS | LYS | TYR | ASN | ALA | TYR | ARG | ||||
| 5 | LYS | ALA | ALA | SER | VAL | ILE | ALA | LYS | TYR | PRO | ||||
| 6 | HIS | LYS | ILE | LYS | SER | GLY | ALA | GLU | ALA | LYS | ||||
| 7 | LYS | LEU | PRO | GLY | VAL | GLY | THR | LYS | ILE | ALA | ||||
| 8 | GLU | LYS | ILE | ASP | GLU | PHE | LEU | ALA | THR | GLY | ||||
| 9 | LYS | LEU | ARG | LYS | LEU | GLU | LYS |
Samples:
sample_one: DNA Polymerase B, [U-100% 13C; U-100% 15N], 2.8 mM; Tris-d11 5 mM; NaCl 400 mM
sample_two: DNA Polymerase B, [U-100% 15N], 4.7 mM; Tris-d11 5 mM; NaCl 400 mM
sample_three: DNA Polymerase B 1.4 mM; Tris-d11 5 mM; NaCl 400 mM
sample_conditions: ionic strength: 0.800 .; pH: 6.7 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | not available | not available | sample_conditions |
| 2D 1H-1H TOCSY | not available | not available | sample_conditions |
| 2D 1H-1H DQF-COSY | not available | not available | sample_conditions |
| 2D 1H-15N HMQC | not available | not available | sample_conditions |
| 2D 1H-15N HMQC-J | not available | not available | sample_conditions |
| 3D 1H-15N-1H NOESY-HMQC | not available | not available | sample_conditions |
| 3D 1H-15N-1H TOCSY-HMQC | not available | not available | sample_conditions |
| 3D 1H-13C-1H TOCSY-HMQC | not available | not available | sample_conditions |
| 3D HNCA | not available | not available | sample_conditions |
| 3D HN(CO)CA | not available | not available | sample_conditions |
| 3D HNCO | not available | not available | sample_conditions |
| 3D 1H-13C-1H NOESY-HMQC | not available | not available | sample_conditions |
Software:
Felix v2.10 - processing and analysis of spectra
NMR spectrometers:
- General Electric GN-500 500 MHz
Related Database Links:
| BMRB | 18267 |
| PDB | |
| DBJ | BAA06099 BAC36630 BAD97257 BAE27405 BAE30399 |
| EMBL | CAD29079 CAD29080 CAD29081 CAD29082 CAD29083 |
| GB | AAA41900 AAA41901 AAA60133 AAB00389 AAB59441 |
| REF | NP_001029936 NP_001244632 NP_001297194 NP_002681 NP_035260 |
| SP | P06746 P06766 Q27958 Q8K409 |
| TPG | DAA14453 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts